Sains Malaysiana 55(9)(2026): 1476-1486

http://doi.org/10.17576/jsm-2026-5509-06

 

Peningkatan Pencuraian Efluen Kilang Kelapa Sawit (POME) Berasid dan Penghasilan Biogas melalui Pengayaan Kultur Pemula

(Improved Acidified Palm Oil Mill Effluent (POME) Digestion and Biogas Production through Starter Culture Enrichment)

 

NAQIBAH BALQIS BINTI BADRULZAMAN1, NAZLINA HAIZA BINTI MOHD YASIN1,*, NUR HAZLIN HAZRIN-CHONG1 & PEER MOHAMED ABDUL2

 

1Jabatan Sains Biologi dan Bioteknologi, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

2Jabatan Kejuruteraan Kimia dan Proses, Fakulti Kejuruteraan dan Alam Bina, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

 

Received: 11 June 2025/Accepted: 24 August 2026

 

*Corresponding author; email: nazlinayasin@ukm.edu.my

 

Abstrak

Efluen kilang kelapa sawit (POME) adalah antara penyumbang terbesar kepada pencemaran alam sekitar kerana Malaysia merupakan antara pengeksport minyak sawit terbesar di dunia. Sistem kolam terbuka untuk rawatan POME merupakan sistem konvensional yang menyumbang kepada pelepasan gas rumah hijau yang tidak terkawal, selain memerlukan masa rawatan yang panjang. Oleh itu, kajian ini bertujuan untuk membangunkan kultur pemula melalui proses pencernaan anaerobik (AD) dengan menggunakan POME berasid bagi memendekkan masa rawatan dan meningkatkan penghasilan biogas dan gas metana. Dalam kajian ini, pengayaan kultur dengan sistem suapan berkala POME berasid dilakukan dengan masa penempatan hidraulik (HRT) selama 30 hari sehingga mencapai kestabilan penghasilan biogas dan gas metana. Hasil kajian menunjukkan berlakunya peningkatan dalam penghasilan biogas dan gas metana antara hari ke-28 hingga 72 semasa proses pengayaan kultur pemula bagi penyediaan kultur metanogenik. Peningkatan gas metana berkait dengan pengurangan kepekatan asid asetik yang menandakan terdapatnya peningkatan aktiviti metanogen dan kestabilan sistem AD. Kesimpulannya, melalui pengayaan kultur metanogen, kandungan biogas dan gas metana meningkat dalam tempoh HRT yang singkat sekali gus mengurangkan pengumpulan asid lemak meruap yang membawa kepada perencatan proses AD.

Kata kunci: Kultur pemula; metanogen; pencernaan anaerobik; POME

 

Abstract

Palm oil mill effluent (POME) is one of the largest contributors to environmental pollution, as Malaysia is one of the world’s largest exporters of palm oil. Open pond systems for POME treatment are conventional systems that contribute to uncontrolled greenhouse gas emissions with longer retention times. Therefore, this study aims to develop starter cultures through anaerobic digestion (AD) using acidified POME to shorten the treatment time and enhance biogas and methane yield. In this study, the enriched culture was fed with the acidic POME with a hydraulic retention time (HRT) of 30 days until the production of biogas and methane gas stabilised. The results indicate that biogas and methane production increased from day 28 to day 72 during the starter culture enrichment process for the preparation of a methanogenic culture. The increase in methane gas is corresponded to the decrease of acetic acid concentration, indicating the methanogens activity and AD system stabilisation.  In conclusion, enriching the methanogen culture led to increased biogas and methane production under a short HRT period while reducing the accumulation of volatile fatty acids, which can otherwise inhibit the AD process.

Keywords: Anaerobic digestion; methanogen; POME; start-up culture

 

REFERENCES

Agostini, S., Bucci, L., Doni, D., Costantini, P., Gupte, A., Müller, B., Sibilla, F., Basaglia, M., Sergio, C., Kougias, P.G., Campanaro, S., Lorenzo, F. & Treu, L. 2024. Bioaugmentation strategies based on bacterial and methanogenic cultures to relieve stress in anaerobic digestion of protein-rich substrates. Renewable Energy 225: 120270. https://doi.org/10.1016/j.renene.2024.120270

Ahmad, S.M., Ismail, A., Wahab, N.A., Salleh, K.M., Kamil, N.N. & Abdullah, N. 2024. Comparative analysis of current biomass utilisation by palm oil mills in Peninsular Malaysia. Journal of Sustainability Science and Management 19: 166-176. https://doi.org/10.46754/jssm.2024.07.009

Aili Hamzah, A.F., Hamzah, M.H., Che Man, H., Jamali, N.S., Siajam, S.I. & Ismail, M.H. 2023. Effect of organic loading on anaerobic digestion of cow dung: Methane production and kinetic study. Heliyon 9(6): e16791. https://doi.org/10.1016/j.heliyon.2023.e16791

Akuma, S.O., Yazdi, S.K. & Eze, U. 2026. A review of resource recovery from palm oil mill effluent (POME): Recent technological innovations and pathways to circular economy. Next Sustainability 7: 100261. https://doi.org/10.1016/j.nxsust.2026.100261

Alrawi, R.A., Ahmad, A., Ismail, N. & Kadir, M.O.A. 2011. Anaerobic co-digestion of palm oil mill effluent with rumen fluid as a co-substrate. Desalination 269(1-3): 50-57. https://doi.org/10.1016/j.desal.2010.10.041

Ao, T-J., Liu, C-G., Sun, Z-Y., Zhao, X-Q., Tang, Y-Q. & Bai, F-W. 2024. Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ. Biotechnology Advances 73: 108372. https://doi.org/10.1016/j.biotechadv.2024.108372

American Public Health Association (APHA). 2017. Standard Methods for the Examination of Water and Wastewater. 23rd ed. Washington DC: American Public Health Association.

Asano, Y. & Kaul, P. 2012. 7.7 Hydrolysis and reverse hydrolysis: Selective nitrile hydrolysis using nitrilase and its related enzymes. Comprehensive Chirality 7: 122-142. https://doi.org/10.1016/B978-0-08-095167-6.00708-4.

Ayua, T.J., Ambrose, I.S. & Omaku, D.S. 2026. Anaerobic digestion of poultry droppings for biogas production: A pilot study of renewable energy technology in the agricultural sector. BMC Environmental Science 3: 7.  https://doi.org/10.1186/s44329-026-00048-8

Aziz, N.I.H. & Hanafiah, M.M. 2018 Anaerobic digestion of palm oil mill effluent (POME) using bio-methane potential (BMP) test. AIP Conference Proceedings 1940: 020026. https://doi.org/10.1063/1.5027941

Badiei, M., Jahim, J.M., Anuar, N. & Sheikh Abdullah, S.R. 2011. Effect of hydraulic retention time on biohydrogen production from palm oil mill effluent in anaerobic sequencing batch reactor. International Journal of Hydrogen Energy 36(10): 5912-5919. https://doi.org/10.1016/j.ijhydene.2011.02.054

Basri, M.F., Yacob, S., Hassan, M.A., Shirai, Y., Wakisaka, M., Zakaria, M.R. & Phang, L.Y. 2010. Improved biogas production from palm oil mill effluent by a scaled-down anaerobic treatment process. World Journal of Microbiology and Biotechnology 26(3): 505-514. https://doi.org/10.1007/s11274-009-0197-x

Carotenuto, C., Guarino, G., Morrone, B. & Minale, M. 2016. Temperature and pH effect on methane production from buffalo manure anaerobic digestion. International Journal of Heat and Technology 34(2): 425-429. https://doi.org/10.18280/ijht.34S233

Chan, Y.J., Chong, M.F. & Law, C.L. 2010. Biological treatment of anaerobically digested palm oil mill effluent (POME) using a lab-scale sequencing batch reactor (SBR). Journal of Environmental Management 91(8): 1738-1746. https://doi.org/10.1016/j.jenvman.2010.03.021

Chen, L., Du, S. & Xie, L. 2021. Effects of pH on ex-situ biomethanation with hydrogenotrophic methanogens under thermophilic and extreme-thermophilic conditions. Journal of Bioscience and Bioengineering 131(2): 168-175. https://doi.org/10.1016/j.jbiosc.2020.09.018

Dareioti, M.A., Dokianakis, S.N., Stamatelatou, K., Zafiri, C. & Kornaros, M. 2009. Biogas production from anaerobic co-digestion of agroindustrial wastewaters under mesophilic conditions in a two-stage process. Desalination 248(1-3): 891-906. https://doi.org/10.1016/j.desal.2008.10.010

Darwin & Cord-Ruwisch, R. 2019. Thermodynamics of anaerobic digestion: Mechanism of suppression on biogas production during acidogenesis.  INMATEH-Agricultural Engineering 57(1): 287-301.

Darwin, Novi Diana, Mardhotillah & Atmadian Pratama. 2021. Anaerobic co-digestion of cow manure and palm oil mill effluent (POME): Assessment of methane production and biodegradation efficiency. International Journal of Design and Nature and Ecodynamics 16(6): 671-676. https://doi.org/10.18280/ijdne.160608

Erguè, T.H., Tezel, U., Guè, E. & Demirer, G.N. 2001. Anaerobic biotransformation and methane generation potential of cheese whey in batch and UASB reactors. Waste Management 21(7): 643-650. https://doi.org/10.1016/S0956-053X(00)00114-8

Erhons, E.K., Odegwa, I.F. & Beckley, I. 2024. Improvement in oxygen demand capacities of palm oil mill effluent from Ujiogba Edo State using some selected bacterial and fungal isolates. Jordan Journal of Earth and Environmental Sciences 15(1): 14-19.

Gu, J.D. 2021. On enrichment culturing and transferring technique. Applied Environmental Biotechnology 6(1): 1-5. https://doi.org/10.26789/AEB.2021.01.001

Hamzah, M.A.F., Abdul, P.M., Mahmod, S.S., Azahar, A.M. & Jahim, J.M. 2020. Performance of anaerobic digestion of acidified palm oil mill effluent under various organic loading rates and temperatures. Water 12(9): 2432. https://doi.org/10.3390/w12092432

Hamzah, M.A.F., Jahim, J.M., Abdul, P.M. & Asis, A.J. 2019. Investigation of temperature effect on start-up operation from anaerobic digestion of acidified palm oil mill effluent. Energies 12(13): 2473.  https://doi.org/10.3390/en12132473

Hanipa, M.A.F., Tiang, M.F., Luthfi, A.A.I., Sajab, M.S., Abu Bakar, M.H., Reungsang, A., Lay, C.H., Wu, S-Y., Kamarudin, K.F. & Abdul, P.M. 2024. Illumination optimization strategies to enhance hydrogen productivity and light conversion efficiency for photo-fermentation by Rhodobacter sphaeroides KKU-PS1 using a concentrated multi-substrate feedstock. International Journal of Hydrogen Energy 88: 418-431. https://doi.org/10.1016/j.ijhydene.2024.09.132

Harnadek, C.M.W., Guilford, N.G.H. & Edwards, E.A. 2015. Chemical oxygen demand analysis of anaerobic digester contents. STEM Fellowship Journal 1: 2-5. https://doi.org/10.17975/sfj-2015-008

Isa, M.H., Wong, L.P., Bashir, M.J.K., Shafiq, N., Kutty, S.R.M., Farooqi, I.H. & Lee, H.C. 2020. Improved anaerobic digestion of palm oil mill effluent and biogas production by ultrasonication pretreatment. Science of The Total Environment 722: 137833. https://doi.org/10.1016/j.scitotenv.2020.137833

Kamyab, H., Chelliapan, S., Din, M.F.M., Rezania, S., Khademi, T. & Kumar, A. 2018. Palm oil mill effluent as an environmental pollutant. InTech. https://doi.org/10.5772/intechopen.75811

Khan, S., Lu, F., Jiang, Q., Jiang, C., Kashif, M. & Shen, P. 2020. Assessment of multiple anaerobic co-digestions and related microbial community of molasses with rice alcohol wastewater. Energies 13(18): 4866. https://doi.org/10.3390/en13184866

Leandro, T., Rodriguez, N., Rojas, P., Sanz, J.L., da Costa, M.S. & Amils, R. 2018. Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt. Heliyon 4(4): e00605. https://doi.org/10.1016/j.heliyon.2018.e00605

Leela, D., Nur, S.M., Yandri, E. & Ariati, R. 2018. Performance of palm oil mill effluent (POME) as biodiesel source based on different ponds. E3S Web of Conferences 67: 02038. 10.1051/e3sconf/20186702038

Liew, W.L., Kassim, M.A., Muda, K., Loh, S.K. & Affam, A.C. 2015. Conventional methods and emerging wastewater polishing technologies for palm oil mill effluent treatment: A review. Journal of Environmental Management 149: 222-235. https://doi.org/10.1016/j.jenvman.2014.10.016

Madhuri, R.J., Saraswathi, M., Gowthami, K., Bhargavi, M., Divya, Y. & Deepika, V. 2019. Chapter 19 - Recent approaches in the production of novel enzymes from environmental samples by enrichment culture and metagenomic approach. In Recent Developments in Applied Microbiology and Biochemistry, edited by Buddolla, V. Massachusetts: Academic Press. hlm. 251-262. https://doi.org/10.1016/B978-0-12-816328-3.00019-2

Mirbagheri, S.A., Bagheri, M., Ehteshami, M., Bagheri, Z. & Pourasghar, M. 2015. Modeling of mixed liquor volatile suspended solids and performance evaluation for a sequencing batch reactor. Journal of Urban and Environmental Engineering 9(1): 54-65. https://doi.org/10.4090/juee.2015.v9n1.054065

Mohammad, S., Baidurah, S., Kobayashi, T., Ismail, N. & Leh, C.P. 2021. Palm oil mill effluent treatment processes - A review. Processes 9(5): 739. https://doi.org/10.3390/pr9050739

Monge, O., Certucha Barragn, M.T. & Almendariz Tapi, F.J. 2013. Microbial biomass in batch and continuous system. In Biomass Now - Sustainable Growth and Use, edited by Matovic, M.D. InTech. https://doi.org/10.5772/55303

Morris, B.E.L., Henneberger, R., Huber, H. & Moissl-Eichinger, C. 2013. Microbial syntrophy: Interaction for the common good. FEMS Microbiology Reviews 37(3): 384-406. https//doi.org/10.1111/1574-6976.12019

Mustapha, S., Ashhuby, B., Rashid, M. & Azni, I. 2003. Start-up strategy of a thermophilic upflow anaerobic filter for treating palm oil mill effluent. Process Safety and Environmental Protection 81(4): 262-266. https://doi.org/10.1205/095758203322299798

Oduor, W.W., Wandera, S.M., Murunga, S.I. & Raude, J.M. 2022. Enhancement of anaerobic digestion by co-digesting food waste and water hyacinth in improving treatment of organic waste and bio-methane recovery. Heliyon 8(9): e10580. https://doi.org/10.1016/j.heliyon.2022.e10580

Ohimain, E.I. & Izah, S.C. 2017. A review of biogas production from palm oil mill effluents using different configurations of bioreactors. Renewable and Sustainable Energy Reviews 7: 242-253. https://doi.org/10.1016/j.rser.2016.11.221

Paulo, L.M., Liu, Y.C., Castilla-Archilla, J., Ramiro-Garcia, J., Hughes, D., Mahony, T., Conall Holohan, B., Wilmes, P. & O’Flaherty, V. 2024. Full-scale study on high-rate low-temperature anaerobic digestion of agro-food wastewater: Process performances and microbial community. Water Science and Technology 90(4): 1239-1249. https: doi.org/10.2166/wst.2024.272 

Sani, K., Kongjan, P., Pakhathirathien, C., Cheirsilp, B., O-Thong, S., Raketh, M., Kana, R. & Jariyaboon, R. 2021. Effectiveness of using two-stage anaerobic digestion to recover bio-energy from high strength palm oil mill effluents with simultaneous treatment. Journal of Water Process Engineering 39: 101661. https://doi.org/10.1016/j.jwpe.2020.101661

Saragih, F.N.A., Priadi, C.R., Adityosulindro, S., Abdillah, A. & Islami, B.B. 2019. The effectiveness of anaerobic digestion process by thermal pre-treatment on food waste as a substrate. IOP Conference Series: Earth and Environmental Science 251: 012014. https://doi.org/10.1088/1755-1315/251/1/012014

Steinmetz, R.L.R., Mezzari, M.P., da Silva, M.L.B., Kunz, A., do Amaral, A.C., Tápparo, D.C. & Soares, H.M. 2016. Enrichment and acclimation of an anaerobic mesophilic microorganism’s inoculum for standardization of BMP assays. Bioresource Technology 219: 21-28. https://doi.org/10.1016/j.biortech.2016.07.031

Sulaiman, A., Tabatabaei, M., Zulkhairi, M., Yusoff, M., Ibrahim, M., Hassan, A. & Shirai, Y. 2010. Accelerated start-up of a semi-commercial digester tank treating palm oil mill effluent with sludge seeding for methane production. World Applied Sciences Journal 8(2): 247-258.

Tiang, M.F., Hanipa, M.A.F., Mahmod, S.S., Zainuddin, M.T., Lutfi, A.A.I., Jahim, J.J., Takriff, M.S., Reungsang, A., Wu, S.Y. & Peer, M.A. 2024. Impact of light spectra on photo-fermentative biohydrogen production by Rhodobacter sphaeroides KKU-PS1. Bioresource Technology 394: 130222. https://doi.org/10.1016/j.biortech.2023.130222

Vohra, B., Fazry, S., Sairi, F. & Othman, B.A. 2019. Effects of medium variation and fermentation time towards the pH level and ethanol content of Kombucha. AIP Conference Proceedings 2111: 040008. https://doi.org/10.1063/1.5111247

Wojcieszak, M., Pyzik, A., Poszytek, K., Krawczyk, P.S., Sobczak, A., Lipinski, L., Roubinek, O., Palige, J., Sklodowska, A. & Drewniak, L. 2017. Adaptation of methanogenic inocula to anaerobic digestion of maize silage. Frontiers in Microbiology 8: 1881. https://doi.org/10.3389/fmicb.2017.01881

Wong, Y.S., Wong, Y-S., Ong, S-A., Lim, K-K. & Lee, H-C. 2011. Acclimatization and performance study of acidogenesis anaerobic degradation process for palm oil mill effluent. 2011 International Conference on Environment and Industrial Innovation 12: 1-5.

Woraruthai, T., Kunno, J., Pongsopon, M., Yansakon, K., Phoopraintra, P., Chantiwas, R., Leartsakulpanich, U., Chaiyen, P. & Wongnate, T. 2020. Identification and cultivation of hydrogenotrophic methanogens from palm oil mill effluent for high methane production. International Journal of Energy Research 44(13): 10058-10070.  https://doi.org/10.1002/er.5618

Yan, Y.J., Li, X., Lu, C.S., Kobayashi, T., Zhen, G.Y., & Hu, Y. 2023. A review on start-up phase optimization of kitchen waste anaerobic digestion. Fermentation 9(7), 603. https://doi.org/10.3390/fermentation9070603

Zainal, B.S., Ahmad, M.A., Danaee, M., Jamadon, N., Mohd, N.S. & Ibrahim, S.I. 2020. Integrated system technology of POME treatment for biohydrogen and biomethane production in Malaysia. Applied Sciences 10(3): 951. https://doi.org/10.3390/app10030951

Zobeashia, S.S.L.T, Abioye, O.P., Ijah, U.J.J., Oyewole, O.A., Aransiola, S.A., Maddela, N.R & Prasad, R. 2025. Design and optimization of anaerobic digestion systems by advanced microbial community dynamics modelling. Discover Chemistry. 2:360. https://doi.org/10.1007/s44371-025-00457-9

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

previous next